Experimental recording of a seminar lecture by Prof. C. Giovanni Galizia (Biology Department, Uni Konstanz) about neurobiology of fruit flies and honey bees with regards to odor sensing. We recommend you download the presentation slides and follow them while listening.

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Sendungsnotizen

• Biology department Uni Konstanz
• Prof. Giovanni Galizia (Zoology & Neurobiology)

Sensory transduction: fruit fly

• Honey bee & fruit fly as model organisms
• Antennal lobe: 1st stop in insect brain for sensory input
• Mushroom body & protocerebrum: further processing, learning & memory
• olfaction (olfactory) = (related to) sense of smell
• G-proteins, their coupled receptors (GPCR) & the signal transduction coupled to those
• several proteins called G_{o, i, alpha, etc.} = olfactory GPCRs
• G-protein coupled signal transduction
• Ligand-gated ion channel

Papers mentioned:

• Sato K, Pellegrino M, Nakagawa T, Nakagawa T, Vosshall LB, et al. (2008) Insect olfactory receptors are heteromeric ligand-gated ion channels. Nature 452: 1002–1006
• Smart R, Kiely A, Beale M, Vargas E, Carraher C, et al. (2008) Drosophila odorant receptors are novel seven transmembrane domain proteins that can signal independently of heterotrimeric G proteins. Insect Biochemistry and Molecular Biology 38: 770–780
• Yao CA, Carlson JR (2010) Role of G-Proteins in Odor-Sensing and CO₂-Sensing Neurons in Drosophila. J Neurosci 30: 4562–4572
• Deng Y, Zhang W, Farhat K, Oberland S, Gisselmann G, et al. (2011) The Stimulatory G_αs Protein Is Involved in Olfactory Signal Transduction in Drosophila. PLoS ONE 6: e18605
• Chatterjee A, Roman G, Hardin PE (2009) G_O contributes to olfactory reception in Drosophila melanogaster. BMC Physiology 9: 22
• Kain P, Chakraborty TS, Sundaram S, Siddiqi O, Rodrigues V, et al. (2008) Reduced Odor Responses from Antennal Neurons of G_qα, Phospholipase Cβ, and rdgA Mutants in Drosophila Support a Role for a Phospholipid Intermediate in Insect Olfactory Transduction. J Neurosci 28: 4745–4755
• Wicher D, Schäfer R, Bauernfeind R, Stensmyr MC, Heller R, et al. (2008) Drosophila odorant receptors are both ligand-gated and cyclic-nucleotide-activated cation channels. Nature 452: 1007–1011

1. Behavior

• GABA_BR = GABA-receptor B; coupled to a K⁺ ion channel via G-proteins
• GABA = γ-Aminobutyric acid (neurotransmitter)

2. In vitro experiments

• Heterologous expression
• HEK cells
• Ryanodine

3. In vivo experiments

• Phasic & tonic response
• Phases of an neuron’s action potential

peptide networks: honey bee

• Interneuron
• Tachykinin & Allatostatin: neuropeptides; not recycled like neurotransmitters
• Kreissl S, Strasser C, Galizia CG (2010) Allatostatin immunoreactivity in the honeybee brain. J Comp Neurol 518: 1391–1417

olfactory processing: a model

• AL = antennal lobe
• LP = lateral protocerebrum
• MB = mushroom bodies

Further reading

• Galizia G, Lledo P-M (2013) Neurosciences – From Molecule to Behavior. Springer Spektrum
• Galizia CG (2014) Olfactory coding in the insect brain: data and conjectures. Eur J Neurosci 39: 1784–1795

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• KNS024 - Wie kam das bloß durch die Ethikkommission?